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phosphorylated gr (ser 211) antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated gr (ser 211) antibody
    (a) Male and female rats were prepared with intravenous (IV) catheters and trained to self-administer (SA) methadone (unit dose: 300 μg/kg/0.1 ml) in 1 h ShA sessions (FR1) and split into ShA and LgA (12 h) groups. We used the unit dose of 300 μg/kg/0.1 ml based on studies that reported that rats dose-dependently self-administered methadone and that methadone substitutes for morphine [21–23]. We used the 300 μg/kg/0.1 ml dose for both sexes based on their similar response to methadone-induced antinociception and hyperalgesia during withdrawal [24]. Once rats in the LgA group significantly escalated their methadone self-administration, rats in both the ShA and LgA groups were acutely administered with mifepristone (0, 30, 60, and 90 mg/kg; intraperitoneal, 90 min before testing) in a within-subjects Latin-square design. (b) Methadone infusions (± SEM) in rats in the ShA and LgA groups. ***p < 0.0001, difference from session 1. ShA: n = 18 (10 males, 8 females). LgA: n = 18 (9 males, 9 females). To confirm that the rats in the LgA group exhibited greater signs of opioid dependence than rats in the ShA group, we tested the rats in the von Frey (VF) test to measure hyperalgesia during spontaneous withdrawal and naloxone-precipitated signs of somatic withdrawal (STW). (c) Phosphorylation of GRs at Ser232 in the CeA in rats in the LgA group relative to rats in the ShA group. The optical densities of total GR bands were normalized to the housekeeping protein GAPDH. Because there was no significant difference in total GR levels between the ShA and LgA groups, we normalized the optical densities of <t>phosphorylated</t> GR bands to the optical densities of total GR bands. ***p < 0.001, difference from ShA. ShA: n = 14 (7 males, 7 females). LgA: n = 12 (6 males, 6 females). (d, e) Methadone infusions (± SEM) in (d) rats in the ShA group and (e) rats in the LgA group. **p < 0.01, ***p = 0.0002, difference from 0 mg/kg (Dunnett’s post hoc comparisons). ShA: n = 18. LgA: n = 18.
    Phosphorylated Gr (Ser 211) Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+gr+(ser+211)+antibody/anti+phospho+tfeb/pmc10406162-153-4-14
    Average 90 stars, based on 1 article reviews
    phosphorylated gr (ser 211) antibody - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Corticosteroid Sensitization Drives Opioid Addiction"

    Article Title: Corticosteroid Sensitization Drives Opioid Addiction

    Journal: Molecular psychiatry

    doi: 10.1038/s41380-022-01501-1

    (a) Male and female rats were prepared with intravenous (IV) catheters and trained to self-administer (SA) methadone (unit dose: 300 μg/kg/0.1 ml) in 1 h ShA sessions (FR1) and split into ShA and LgA (12 h) groups. We used the unit dose of 300 μg/kg/0.1 ml based on studies that reported that rats dose-dependently self-administered methadone and that methadone substitutes for morphine [21–23]. We used the 300 μg/kg/0.1 ml dose for both sexes based on their similar response to methadone-induced antinociception and hyperalgesia during withdrawal [24]. Once rats in the LgA group significantly escalated their methadone self-administration, rats in both the ShA and LgA groups were acutely administered with mifepristone (0, 30, 60, and 90 mg/kg; intraperitoneal, 90 min before testing) in a within-subjects Latin-square design. (b) Methadone infusions (± SEM) in rats in the ShA and LgA groups. ***p < 0.0001, difference from session 1. ShA: n = 18 (10 males, 8 females). LgA: n = 18 (9 males, 9 females). To confirm that the rats in the LgA group exhibited greater signs of opioid dependence than rats in the ShA group, we tested the rats in the von Frey (VF) test to measure hyperalgesia during spontaneous withdrawal and naloxone-precipitated signs of somatic withdrawal (STW). (c) Phosphorylation of GRs at Ser232 in the CeA in rats in the LgA group relative to rats in the ShA group. The optical densities of total GR bands were normalized to the housekeeping protein GAPDH. Because there was no significant difference in total GR levels between the ShA and LgA groups, we normalized the optical densities of phosphorylated GR bands to the optical densities of total GR bands. ***p < 0.001, difference from ShA. ShA: n = 14 (7 males, 7 females). LgA: n = 12 (6 males, 6 females). (d, e) Methadone infusions (± SEM) in (d) rats in the ShA group and (e) rats in the LgA group. **p < 0.01, ***p = 0.0002, difference from 0 mg/kg (Dunnett’s post hoc comparisons). ShA: n = 18. LgA: n = 18.
    Figure Legend Snippet: (a) Male and female rats were prepared with intravenous (IV) catheters and trained to self-administer (SA) methadone (unit dose: 300 μg/kg/0.1 ml) in 1 h ShA sessions (FR1) and split into ShA and LgA (12 h) groups. We used the unit dose of 300 μg/kg/0.1 ml based on studies that reported that rats dose-dependently self-administered methadone and that methadone substitutes for morphine [21–23]. We used the 300 μg/kg/0.1 ml dose for both sexes based on their similar response to methadone-induced antinociception and hyperalgesia during withdrawal [24]. Once rats in the LgA group significantly escalated their methadone self-administration, rats in both the ShA and LgA groups were acutely administered with mifepristone (0, 30, 60, and 90 mg/kg; intraperitoneal, 90 min before testing) in a within-subjects Latin-square design. (b) Methadone infusions (± SEM) in rats in the ShA and LgA groups. ***p < 0.0001, difference from session 1. ShA: n = 18 (10 males, 8 females). LgA: n = 18 (9 males, 9 females). To confirm that the rats in the LgA group exhibited greater signs of opioid dependence than rats in the ShA group, we tested the rats in the von Frey (VF) test to measure hyperalgesia during spontaneous withdrawal and naloxone-precipitated signs of somatic withdrawal (STW). (c) Phosphorylation of GRs at Ser232 in the CeA in rats in the LgA group relative to rats in the ShA group. The optical densities of total GR bands were normalized to the housekeeping protein GAPDH. Because there was no significant difference in total GR levels between the ShA and LgA groups, we normalized the optical densities of phosphorylated GR bands to the optical densities of total GR bands. ***p < 0.001, difference from ShA. ShA: n = 14 (7 males, 7 females). LgA: n = 12 (6 males, 6 females). (d, e) Methadone infusions (± SEM) in (d) rats in the ShA group and (e) rats in the LgA group. **p < 0.01, ***p = 0.0002, difference from 0 mg/kg (Dunnett’s post hoc comparisons). ShA: n = 18. LgA: n = 18.

    Techniques Used: Phospho-proteomics

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    Bioprocessing:

    Article Title: Genistein Directly Represses the Phosphorylation of STAT5 in Lactating Mammary Epithelial Cells
    Article Snippet: .. Rabbit polyclonal antibodies against AKT (Cell Signaling Technology, Danvers, MA, USA, 1:1200), phosphorylated AKT (pAKT; Ser473, Cell Signaling Technology, 1:1200), mTOR (Cell Signaling Technology, 1:1200), phosphorylated mTOR (pmTOR; Ser2448, Cell Signaling Technology, 1:1200), GR (Sigma-Aldrich, St. Louis, MO, USA, 1:1000), phosphorylated GR (pGR; Ser211, Cell Signaling Technology, 1:1000), JAK2 (Cell Signaling Technology, 1:750), phosphorylated JAK2 (pJAK2; Tyr1007/1008, Cell Signaling Technology, 1:750), STAT5 (Cell Signaling Technology, 1:1000), phosphorylated STAT5 (pSTAT5; Tyr694, Cell Signaling Technology, 1:1000), EGFR (Cell Signaling Technology, 1:1000), phosphorylated EGFR (pEGFR; Try1068, Cell Signaling Technology, 1:1000), p38 (Cell Signaling Technology, 1:1000), phosphorylated p38 (pp38; Thr180/Tyr182, Cell Signaling Technology, 1:1000), MEK1/2 (Cell Signaling Technology, 1:1000), phosphorylated MEK1/2 (pMEK1/2; Ser217/221, Cell Signaling Technology, 1:1000), ERK1/2 (Cell Signaling Technology, 1:1000), phosphorylated ERK1/2 (pERK1/2; Thr202/Tyr204, Cell Signaling Technology, 1:1000), JNK (Cell Signaling Technology, 1:1000), phosphorylated JNK (pJNK; Thr183/Tyr185, Cell Signaling Technology, 1:1000), ERα (Sigma-Aldrich, 1:500), phosphorylated ERα (pERα; Ser167, Sigma-Aldrich, 1:750), ERβ (Abcam, Cambridge, UK, 1:1000), mouse monoclonal antibodies against β-actin (Sigma-Aldrich, 1:20,000), lamin A/C (Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:1000), α-tubulin (Sigma-Aldrich, 1:1000), and a goat polyclonal antibody against β-casein (Santa Cruz Biotechnology, 1:1000) were purchased and stored at −20 °C until use. ..

    Incubation:

    Article Title: Glucocorticoids activate Yes-associated protein in human vocal fold fibroblasts.
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton-X, and then incubated in phosphate buffered saline containing 0.05% tween-20 and 1% bovine serum albumin for 30 min. Primary antibodies included anti-TAZ (1: 200; #HPA007415, Atlas Antibodies, Stockholm, Sweden), YAP (1:100; sc101199, Santa Cruz Biotechnology, Dallas, TX), GR (1:150; #3660, Cell Signaling Technology, Danvers, MA), Ser211-phosphorylated GR (1:150; #4161, Cell Signaling Technology) antibodies. .. Alexa Fluor 488- conjugated donkey anti-rabbit IgG and Alexa Fluor 555-conjugated donkey anti-mouse IgG antibodies were then employed (Thermo Scientific, Waltham, MA).

    Saline:

    Article Title: Glucocorticoids activate Yes-associated protein in human vocal fold fibroblasts.
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton-X, and then incubated in phosphate buffered saline containing 0.05% tween-20 and 1% bovine serum albumin for 30 min. Primary antibodies included anti-TAZ (1: 200; #HPA007415, Atlas Antibodies, Stockholm, Sweden), YAP (1:100; sc101199, Santa Cruz Biotechnology, Dallas, TX), GR (1:150; #3660, Cell Signaling Technology, Danvers, MA), Ser211-phosphorylated GR (1:150; #4161, Cell Signaling Technology) antibodies. .. Alexa Fluor 488- conjugated donkey anti-rabbit IgG and Alexa Fluor 555-conjugated donkey anti-mouse IgG antibodies were then employed (Thermo Scientific, Waltham, MA).



    Similar Products

    phosphorylated gr (ser 211) antibody  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc phosphorylated gr (ser 211) antibody
    (a) Male and female rats were prepared with intravenous (IV) catheters and trained to self-administer (SA) methadone (unit dose: 300 μg/kg/0.1 ml) in 1 h ShA sessions (FR1) and split into ShA and LgA (12 h) groups. We used the unit dose of 300 μg/kg/0.1 ml based on studies that reported that rats dose-dependently self-administered methadone and that methadone substitutes for morphine [21–23]. We used the 300 μg/kg/0.1 ml dose for both sexes based on their similar response to methadone-induced antinociception and hyperalgesia during withdrawal [24]. Once rats in the LgA group significantly escalated their methadone self-administration, rats in both the ShA and LgA groups were acutely administered with mifepristone (0, 30, 60, and 90 mg/kg; intraperitoneal, 90 min before testing) in a within-subjects Latin-square design. (b) Methadone infusions (± SEM) in rats in the ShA and LgA groups. ***p < 0.0001, difference from session 1. ShA: n = 18 (10 males, 8 females). LgA: n = 18 (9 males, 9 females). To confirm that the rats in the LgA group exhibited greater signs of opioid dependence than rats in the ShA group, we tested the rats in the von Frey (VF) test to measure hyperalgesia during spontaneous withdrawal and naloxone-precipitated signs of somatic withdrawal (STW). (c) Phosphorylation of GRs at Ser232 in the CeA in rats in the LgA group relative to rats in the ShA group. The optical densities of total GR bands were normalized to the housekeeping protein GAPDH. Because there was no significant difference in total GR levels between the ShA and LgA groups, we normalized the optical densities of <t>phosphorylated</t> GR bands to the optical densities of total GR bands. ***p < 0.001, difference from ShA. ShA: n = 14 (7 males, 7 females). LgA: n = 12 (6 males, 6 females). (d, e) Methadone infusions (± SEM) in (d) rats in the ShA group and (e) rats in the LgA group. **p < 0.01, ***p = 0.0002, difference from 0 mg/kg (Dunnett’s post hoc comparisons). ShA: n = 18. LgA: n = 18.
    Phosphorylated Gr (Ser 211) Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+gr+(ser+211)+antibody/anti+phospho+tfeb/pmc10406162-153-4-14
    Average 90 stars, based on 1 article reviews
    phosphorylated gr (ser 211) antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    ser 211-specific phosphorylated forms of gr antibody  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc ser 211-specific phosphorylated forms of gr antibody
    (a) Male and female rats were prepared with intravenous (IV) catheters and trained to self-administer (SA) methadone (unit dose: 300 μg/kg/0.1 ml) in 1 h ShA sessions (FR1) and split into ShA and LgA (12 h) groups. We used the unit dose of 300 μg/kg/0.1 ml based on studies that reported that rats dose-dependently self-administered methadone and that methadone substitutes for morphine [21–23]. We used the 300 μg/kg/0.1 ml dose for both sexes based on their similar response to methadone-induced antinociception and hyperalgesia during withdrawal [24]. Once rats in the LgA group significantly escalated their methadone self-administration, rats in both the ShA and LgA groups were acutely administered with mifepristone (0, 30, 60, and 90 mg/kg; intraperitoneal, 90 min before testing) in a within-subjects Latin-square design. (b) Methadone infusions (± SEM) in rats in the ShA and LgA groups. ***p < 0.0001, difference from session 1. ShA: n = 18 (10 males, 8 females). LgA: n = 18 (9 males, 9 females). To confirm that the rats in the LgA group exhibited greater signs of opioid dependence than rats in the ShA group, we tested the rats in the von Frey (VF) test to measure hyperalgesia during spontaneous withdrawal and naloxone-precipitated signs of somatic withdrawal (STW). (c) Phosphorylation of GRs at Ser232 in the CeA in rats in the LgA group relative to rats in the ShA group. The optical densities of total GR bands were normalized to the housekeeping protein GAPDH. Because there was no significant difference in total GR levels between the ShA and LgA groups, we normalized the optical densities of <t>phosphorylated</t> GR bands to the optical densities of total GR bands. ***p < 0.001, difference from ShA. ShA: n = 14 (7 males, 7 females). LgA: n = 12 (6 males, 6 females). (d, e) Methadone infusions (± SEM) in (d) rats in the ShA group and (e) rats in the LgA group. **p < 0.01, ***p = 0.0002, difference from 0 mg/kg (Dunnett’s post hoc comparisons). ShA: n = 18. LgA: n = 18.
    Ser 211 Specific Phosphorylated Forms Of Gr Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+gr+(ser+211)+antibody/anti+phospho+tfeb/pmc03617099-53-38-41
    Average 90 stars, based on 1 article reviews
    ser 211-specific phosphorylated forms of gr antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) Male and female rats were prepared with intravenous (IV) catheters and trained to self-administer (SA) methadone (unit dose: 300 μg/kg/0.1 ml) in 1 h ShA sessions (FR1) and split into ShA and LgA (12 h) groups. We used the unit dose of 300 μg/kg/0.1 ml based on studies that reported that rats dose-dependently self-administered methadone and that methadone substitutes for morphine [21–23]. We used the 300 μg/kg/0.1 ml dose for both sexes based on their similar response to methadone-induced antinociception and hyperalgesia during withdrawal [24]. Once rats in the LgA group significantly escalated their methadone self-administration, rats in both the ShA and LgA groups were acutely administered with mifepristone (0, 30, 60, and 90 mg/kg; intraperitoneal, 90 min before testing) in a within-subjects Latin-square design. (b) Methadone infusions (± SEM) in rats in the ShA and LgA groups. ***p < 0.0001, difference from session 1. ShA: n = 18 (10 males, 8 females). LgA: n = 18 (9 males, 9 females). To confirm that the rats in the LgA group exhibited greater signs of opioid dependence than rats in the ShA group, we tested the rats in the von Frey (VF) test to measure hyperalgesia during spontaneous withdrawal and naloxone-precipitated signs of somatic withdrawal (STW). (c) Phosphorylation of GRs at Ser232 in the CeA in rats in the LgA group relative to rats in the ShA group. The optical densities of total GR bands were normalized to the housekeeping protein GAPDH. Because there was no significant difference in total GR levels between the ShA and LgA groups, we normalized the optical densities of phosphorylated GR bands to the optical densities of total GR bands. ***p < 0.001, difference from ShA. ShA: n = 14 (7 males, 7 females). LgA: n = 12 (6 males, 6 females). (d, e) Methadone infusions (± SEM) in (d) rats in the ShA group and (e) rats in the LgA group. **p < 0.01, ***p = 0.0002, difference from 0 mg/kg (Dunnett’s post hoc comparisons). ShA: n = 18. LgA: n = 18.

    Journal: Molecular psychiatry

    Article Title: Corticosteroid Sensitization Drives Opioid Addiction

    doi: 10.1038/s41380-022-01501-1

    Figure Lengend Snippet: (a) Male and female rats were prepared with intravenous (IV) catheters and trained to self-administer (SA) methadone (unit dose: 300 μg/kg/0.1 ml) in 1 h ShA sessions (FR1) and split into ShA and LgA (12 h) groups. We used the unit dose of 300 μg/kg/0.1 ml based on studies that reported that rats dose-dependently self-administered methadone and that methadone substitutes for morphine [21–23]. We used the 300 μg/kg/0.1 ml dose for both sexes based on their similar response to methadone-induced antinociception and hyperalgesia during withdrawal [24]. Once rats in the LgA group significantly escalated their methadone self-administration, rats in both the ShA and LgA groups were acutely administered with mifepristone (0, 30, 60, and 90 mg/kg; intraperitoneal, 90 min before testing) in a within-subjects Latin-square design. (b) Methadone infusions (± SEM) in rats in the ShA and LgA groups. ***p < 0.0001, difference from session 1. ShA: n = 18 (10 males, 8 females). LgA: n = 18 (9 males, 9 females). To confirm that the rats in the LgA group exhibited greater signs of opioid dependence than rats in the ShA group, we tested the rats in the von Frey (VF) test to measure hyperalgesia during spontaneous withdrawal and naloxone-precipitated signs of somatic withdrawal (STW). (c) Phosphorylation of GRs at Ser232 in the CeA in rats in the LgA group relative to rats in the ShA group. The optical densities of total GR bands were normalized to the housekeeping protein GAPDH. Because there was no significant difference in total GR levels between the ShA and LgA groups, we normalized the optical densities of phosphorylated GR bands to the optical densities of total GR bands. ***p < 0.001, difference from ShA. ShA: n = 14 (7 males, 7 females). LgA: n = 12 (6 males, 6 females). (d, e) Methadone infusions (± SEM) in (d) rats in the ShA group and (e) rats in the LgA group. **p < 0.01, ***p = 0.0002, difference from 0 mg/kg (Dunnett’s post hoc comparisons). ShA: n = 18. LgA: n = 18.

    Article Snippet: The primary antibodies were phosphorylated GR (Ser 211 ) antibody (1:500, catalog no. 4161, Cell Signaling, Danvers, MA, USA), GR monoclonal antibody (1:500, catalog no. MA1–510, Invitrogen, Waltham, MA, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2500, catalog no. AB9485, Abcam, Cambridge, MA, USA).

    Techniques: Phospho-proteomics